蛋白质聚合产生的力
Models for how polymerization of actin filaments can produce enough force to push forward the leading edge of a cell at the speeds seen in living cells.
运动蛋白的介绍
Molecular motor proteins are fascinating enzymes that power much of the movement performed by living organisms. In the first part of this lecture, I will provide an overview of the motors that move along cytoskeletal tracks (kinesin and dynein which move along microtubules and myosin which moves along actin). The main focus of this lecture is on how motor proteins work. How does a nanoscale protein convert energy from ATP hydrolysis into unidirectional motion and force production? What tools do we have at our disposal to study them? The first part of the lecture will focus on these questions for kinesin (a microtubule-based motor) and myosin (an actin-based motor), since they have been the subject of extensive studies and good models for their mechanisms have emerged. I conclude by discussing the importance of understanding motor proteins for human disease, in particular illustrating a recent biotechnology effort from Cytokinetics, Inc. to develop drugs that activate cardiac myosins to improve cardiac contractility in patients suffering from heart failure. The first part of the lecture is directed to a general audience or a beginning graduate class.
In the second part of this lecture, I will discuss our laboratories current work on the mechanism of movement by dynein, a motor protein about which we still know very little. This is a research story in progress, where some advances have been made. However, much remains to be done in order to understand how this motor works.
The third (last) part of the lecture is on mitosis, the process by which chromosomes are aligned and then segregated during cell division. I will describe our efforts to find new proteins that are important for mitosis through a high throughput RNAi screen. I will discuss how we technically executed the screen and then focus on new proteins that are we discovered that are involved in generating the microtubules that compose the mitotic spindle. I also discuss the medical importance of studying mitosis, including the development of drugs targeted to mitotic motor proteins, which are currently undergoing testing in clinical trials.
单分子分析方法研究运动蛋白
Molecular motor proteins are fascinating enzymes that power much of the movement performed by living organisms. In the first part of this lecture, I will provide an overview of the motors that move along cytoskeletal tracks (kinesin and dynein which move along microtubules and myosin which moves along actin). The main focus of this lecture is on how motor proteins work. How does a nanoscale protein convert energy from ATP hydrolysis into unidirectional motion and force production? What tools do we have at our disposal to study them? The first part of the lecture will focus on these questions for kinesin (a microtubule-based motor) and myosin (an actin-based motor), since they have been the subject of extensive studies and good models for their mechanisms have emerged. I conclude by discussing the importance of understanding motor proteins for human disease, in particular illustrating a recent biotechnology effort from Cytokinetics, Inc. to develop drugs that activate cardiac myosins to improve cardiac contractility in patients suffering from heart failure. The first part of the lecture is directed to a general audience or a beginning graduate class.
In the second part of this lecture, I will discuss our laboratories current work on the mechanism of movement by dynein, a motor protein about which we still know very little. This is a research story in progress, where some advances have been made. However, much remains to be done in order to understand how this motor works.
The third (last) part of the lecture is on mitosis, the process by which chromosomes are aligned and then segregated during cell division. I will describe our efforts to find new proteins that are important for mitosis through a high throughput RNAi screen. I will discuss how we technically executed the screen and then focus on new proteins that are we discovered that are involved in generating the microtubules that compose the mitotic spindle. I also discuss the medical importance of studying mitosis, including the development of drugs targeted to mitotic motor proteins, which are currently undergoing testing in clinical trials.
GTP结合蛋白作为调节分子
When a growth factor binds to the plasma membrane of a quiescent cell, an intracellular signaling pathway is activated telling the cell to begin growing. A key molecule in this signaling pathway is the GTP-binding protein, or G-protein, Ras. Ras can act as an on-off switch telling the cell to grow or not. In its inactive form, Ras is bound to GDP while in its active form it is bound to GTP. This exchange of nucleotides is catalysed by guanine nucleotide-exchange-factors (GEFs). The return to the inactive state occurs through the GTPase reaction, which is accelerated by GTPase-activating proteins (GAPs). In Part 1 of his talk, Dr. Wittinghofer explains how solving the three-dimensional structure of Ras, and other G-proteins, allowed him to understand the conserved mechanism by which G-proteins can act as switches. The structure also identified domains unique to each G-protein that provide the specificity for downstream signals.
Western Blot 第2阶段：蛋白电泳(SDS-PAGE)
Novus Biologicals Visual Protocols: In phase 2 of the western blot procedure, you will learn how to load a gel and separate the proteins through electrophoresis, based upon protein weight. Additional help can be found in the support section of http://www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.
蛋白质组学在精准医疗中的应用
基因→ mRNA→蛋白质，三位一体，构成了遗传信息的流程图，这即是传统的中心法则。基因是遗传信息的源头，而功能性蛋白是基因功能的执行体。在疾病预防、疾病诊断、疾病治疗等方面都有着很大的临床应用价值，是精准医学的重要组成部分。
讲座主要内容：
1)国内外精准医学研究的内涵和现状；
2)精准医学与蛋白质组学的关系；
3)精准医疗时代的Biomarker的研究方法和思路；
4)蛋白质组学在疾病发生发展机制、疾病生物标志物发现以及临床应用方面的实例。
基因合成与蛋白表达
目前生物科研领域中有超过一半以上的研究内容是与蛋白表达相关的，我们对于蛋白表达的了解又有多少呢？本期演讲中包含了蛋白表达的基础知识，常用的蛋白表达系统以及各自的优劣势，如何根据自己的研究目的去选择合适的蛋白表达系统，如何巧妙将基因合成运用于您的蛋白表达设计使得您事半功倍，并且会为您详细解释为什么通过密码子优化能够有助于您下游的蛋白表达，让您在蛋白表达的科研之路上不再是“小白”。
【GE技术大咖秀】2016第三弹之重组标签蛋白纯化策略及方法
本次讲解将与大家一起分享关于重组标签蛋白的纯化策略以及可能会涉及到的一些常用的技术方法。内容主要分为两部分：首先与大家一起了解一下什么是重组标签蛋白，他的表达和纯化的基本流程有哪些，一般会采用什么样的方式进行纯化。接下来，与大家分享一些重组标签蛋白纯化方面的新的解决方案。
核蛋白：核建筑积木
核蛋白是V型中间丝蛋白,核纤层的内核保护分子之间的接口, 的核纤层蛋白是核纤层蛋白。核纤层蛋白二聚体是高阶层组件的积木。核蛋白的结构特性和功能、核大小和形状的决定因素、纺锤体的组装、DNA合成、DNA损伤修复、核膜结构的支持、核孔的支撑与定位、染色质锚固和组织、核纤层蛋白DNA复制中断组织块.、核纤层蛋白网络中断改变DNA的复制的组织与功能、细胞增殖与衰老、罕见的人类疾病提供意想不到的见解，核纤层蛋白的乐趣；哈钦森- Gilford早衰证、核纤层蛋白A基因突变导致患者产生早老蛋白核皱缩、女性早衰细胞中多聚性构损失.、核纤层蛋白A基因突变数目惊人，法尼基转移、抑制剂（伴）在第一个早衰症治疗的试验参与者、早衰细胞细胞核的分离的A和B型核纤层蛋白在这表明他们在滤过泡形成独立的结构。诱导的泡核纤层蛋白A丰富并附上主要的常染色质。西北大学费因伯格医学和海洋生物学院已开始在不同的功能的研究，如果A型和B型非均匀性校正，LB1和lmnb1 WI-38细胞衰老过程中基因表达的损失， LB1沉默减慢增殖和诱导早衰。