石蜡包埋切片样本的RNAscope®检测操作步骤：棕色显色方法
对于石蜡包埋切片（FFPE）类型的样本，使用RNAscope®技术进行RNA原位定量检测分析，结果以棕色化学显色法显示。本视频对该实验进行了分步详细描述，供大家在实验开始时参考学习。
RNAscope®®原位定量专利技术由美国ACD公司(Advanced Cell Diagnostics, Inc., California, USA)开发，通过专利的双“Z”探针设计，使RNA原位杂交具有高度特异性、RNA单分子检测的敏感性并兼容自动化高通量分析，能够在单细胞水平同时定量多个RNA的表达。该视频由ACD公司制作，详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号（ACD_China）咨询。
GE：重组标签蛋白纯化策略与方法
生物分子的分离纯化是获得有效生物产品的重要环节之一。在纯化过程中需要考虑目的物性质，合理选择及组合具有各自特点及优势的层析技术，才能达到最优的纯化效果，满足目标产品产量、纯度等多方面的要求。自世界上第一款凝胶过滤填料诞生开始， GE公司作为蛋白分离纯化领域的专家，始终和您站在一起。“如何综合安排纯化策略？如何有机运用纯化方法？如何灵活优化纯化条件？”在这个讲座中，我们将探讨这些重组标签蛋白纯化工作中的重点和难点。
单分子分析方法研究运动蛋白
Molecular motor proteins are fascinating enzymes that power much of the movement performed by living organisms. In the first part of this lecture, I will provide an overview of the motors that move along cytoskeletal tracks (kinesin and dynein which move along microtubules and myosin which moves along actin). The main focus of this lecture is on how motor proteins work. How does a nanoscale protein convert energy from ATP hydrolysis into unidirectional motion and force production? What tools do we have at our disposal to study them? The first part of the lecture will focus on these questions for kinesin (a microtubule-based motor) and myosin (an actin-based motor), since they have been the subject of extensive studies and good models for their mechanisms have emerged. I conclude by discussing the importance of understanding motor proteins for human disease, in particular illustrating a recent biotechnology effort from Cytokinetics, Inc. to develop drugs that activate cardiac myosins to improve cardiac contractility in patients suffering from heart failure. The first part of the lecture is directed to a general audience or a beginning graduate class.
In the second part of this lecture, I will discuss our laboratories current work on the mechanism of movement by dynein, a motor protein about which we still know very little. This is a research story in progress, where some advances have been made. However, much remains to be done in order to understand how this motor works.
The third (last) part of the lecture is on mitosis, the process by which chromosomes are aligned and then segregated during cell division. I will describe our efforts to find new proteins that are important for mitosis through a high throughput RNAi screen. I will discuss how we technically executed the screen and then focus on new proteins that are we discovered that are involved in generating the microtubules that compose the mitotic spindle. I also discuss the medical importance of studying mitosis, including the development of drugs targeted to mitotic motor proteins, which are currently undergoing testing in clinical trials.
高压蒸汽灭菌：最可靠有效的灭菌方法
Niles North STEM lab instructional video on how to use the Autoclave device.
【GE技术大咖秀】2016第三弹之重组标签蛋白纯化策略及方法
本次讲解将与大家一起分享关于重组标签蛋白的纯化策略以及可能会涉及到的一些常用的技术方法。内容主要分为两部分：首先与大家一起了解一下什么是重组标签蛋白，他的表达和纯化的基本流程有哪些，一般会采用什么样的方式进行纯化。接下来，与大家分享一些重组标签蛋白纯化方面的新的解决方案。
脂质组学的研究进展与分析方法
脂质是一类疏水性或两性小分子。脂质组学（Lipidomics）是一种基于高通量分析技术，系统性解析生物体脂质组成与表达变化的研究模式。 脂质组学分析，可以高效地研究脂类家族、脂质分子在各种生物过程中的改变与功能，进而阐明相关的生物活动机制与原理。 本次课程中科新生命严峻博士就“ 脂质组学的研究进展与分析方法”为题向大家介绍脂质组学的最新研究进展，了解SCI文献中常用的脂质组学分析技术与方法及SCI文献脂质组学的研究思路与文章架构。
非编码RNA研究方法
石蜡切片制备及其免疫组化染色方法介绍
无论是在教学科研中，还是在病理诊断领域，病理切片染色是不可缺少的环节。其规范操作流程包括取材、固定、脱水、包埋、切片、染色、结果分析等，每个步骤都有各自的难点和注意事项。本次讲座主要涉及组织预处理方法和注意事项，以及石蜡切片的免疫组织化学染色技术的原理、操作流程和结果分析等经验分享。
病毒去除/灭活的方法及工艺的选择
目前动物源性病毒感染人类的风险性极高，潜在的医源性感染性问题变得日益突出。近些年为提高制品的生物安全性，国内外药品申报对于病毒清除验证的研究也越来越重视，本次讲座就病毒纯化、病毒灭活、病毒去除方法以及国内外申报差异等方面内容进行阐述，帮助大家更好的选择病毒清除验证研究的工艺。