Study the pathological features of diseases using indUCed pluripotent stem cells derived form patient's somatic cells
The limited experimental access to disease-affected human tissues has severely impeded the elUCidating of molecular mechanisms underlying disease development. Generation of indUCed pluripotent stem cells (iPSCs) by over-expression of defined transcription factors in somatic cells, in particular in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkers as well as therapeutic medicines. Recently, many research groups have independently reported that patient-specific iPSC-derived cells recapitulated multiple features of pathological events of a particular disease, offering experimental evidence of utilizing patient-specific iPSCs to model diseases and reevaluate the current therapies. We have derived iPSC lines using somatic cells of patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to use these iPSC lines to recapitulate the pathological features of the diseases. Our results show that patient's specific iPSC lines provide good opportunity to study the development and treatment of diseases.
sRNA IndUCes the Large-scale Transdetermination of Mesenchymal Stem Cells into Hematopoietic Stem Cells in Human.
Mesenchymal stem cells (MSCs) can differentiate into cells of bone, endothelium, adipose tissue, cartilage, muscle, and brain. However, whether they can transdeterminate into hematopoietic stem cells (HSCs) remains unsolved. We report here that a subpopulation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could differentiate into hematopoietic stem cells (CD150+/CD133+/CD34+) and their descending blood cells in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectively delivered into MSCs by a novel peptide means. These indUCed MSC-HSCs could form different types of hematopoietic colonies as nature-occurring HSCs did. Upon transplantation into sublethally irradiated NOD/SCID mice, these MSC-HSCs engrafted and differentiated into all hematopoietic lineages sUCh as erythrocytes, lymphocytes, myelocytes and thrombocyte. More importantly, these indUCed HSCs could sUCcessfully engraft and effectively function in patients with severe aplastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was indUCed by acetylation of histone proteins and activation of many transcriptional factors. Together, our findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of blood diseases and artificial stem cell-made blood.
Study the pathological features of diseases using indUCed pluripotent stem cells derived form patient's somatic cells
The limited experimental access to disease-affected human tissues has severely impeded the elUCidating of molecular mechanisms underlying disease development. Generation of indUCed pluripotent stem cells (iPSCs) by over-expression of defined transcription factors in somatic cells, in particular in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkers as well as therapeutic medicines. Recently, many research groups have independently reported that patient-specific iPSC-derived cells recapitulated multiple features of pathological events of a particular disease, offering experimental evidence of utilizing patient-specific iPSCs to model diseases and reevaluate the current therapies. We have derived iPSC lines using somatic cells of patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to use these iPSC lines to recapitulate the pathological features of the diseases. Our results show that patient's specific iPSC lines provide good opportunity to study the development and treatment of diseases.
HUC-MSCS移植治疗T2DM的临床试验
来自中国医科大学四平医院的孙丽老师讲述了HUC-MSCS移植治疗T2DM的临床试验，主要内容包括：
一、我国糖尿病现状
二、慢性并发症是DM致死致残
三、传统治疗缺陷--替代/补充
四、干细胞移植治疗糖尿病的国内外现状
五、MSCs移植治疗DM机制等
Controlling the Cell Cycle: IntrodUCtion - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) Part 1: Controlling the Cell Cycle: IntrodUCtion
Cells reprodUCe by duplicating their chromosomes and other components and then distributing them into a pair of genetically identical daughter cells. This series of events is called the cell cycle. In the first part of this lecture, I provide a general overview of the cell-cycle control system, a complex regulatory network that guides the cell through the steps of cell division. I briefly describe the major components of this regulatory system and how they fit together to form a series of biochemical switches that trigger cell-cycle events at the correct time and in the correct order.
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IntrodUCing the EnSight™ Multimode Plate Reader from PerkinElmer
PerkinElmer's EnSight Multimode Plate Reader is the first benchtop system to offer well-imaging alongside label-free and labeled detection technologies - for a whole new perspective on your research. For more information, please visit the EnSight website - http://bit.ly/T4IDPh
IntrodUCing the Opera Phenix™ High Content Screening System
IntrodUCing Opera Phenix™, a next-generation, confocal, high content screening system designed for high-throughput, phenotypic screening and assays using complex disease models, sUCh as primary cells and microtissues. Find out more at http://bit.ly/1eZM4Ok.
PureBlu™ Hoechst 33342 NUClear Staining Dye for Live Cells - A Fast Approach to Staining NUClei
This brief tutorial demonstrates the use of the PureBlu Hoechst 33342 Dye with the ZOE™ Fluorescent Cell Imager for routine nUClear staining in fluorescence microscopy and cell imaging applications.
IncUCyte®活细胞成像和分析平台助力复杂肿瘤细胞实时动态研究
目前，很多细胞检测方法仍然采用“终点法”，只能获取单个时间点的结果，而无法了解细胞的动态变化。在此，我们介绍一种活细胞实时成像分析法，可以对培养箱中的细胞生物学进行长期动态监测和自动分析，如免疫细胞对肿瘤细胞的动态杀伤过程，细胞周期的进展，细胞代谢的动态变化等，而避免外界环境因素对细胞检测结果的影响。 凭借IncUCyte活细胞分析系统这一强大平台，任何细胞生物学家都可以动态了解所培养细胞的健康状况、形态、运动和功能，以便快速获得新的见解，推动研究进展！赛多利斯新型IncUCyte® SX5活细胞分析系统采用独特的光学模块（专利申请中），整合了长波长NIR光源，以减少长期活细胞实验的光毒性。该系统配置多达五个不同的荧光通道，单次实验中可使用多达三个荧光通道和HD相位，从而使科学家获得更多的数据和信息。
IncUCyte活细胞分析系统加速细胞治疗开发
针对细胞治疗的开发，IncUCyte® 可以提供提供一系列免疫细胞功能分析的完整解决方案，从T 细胞活化、T 细胞杀伤，细胞吞噬，到肿瘤球生长等均可实现实时可视化和自动化..