从外周血中提取DRAbek基因组DNA
DRAbek genomic DNA extRActions from peripheRAl blood. Employing a novel DNA extRAction procedure adapted from DRAbek [Biomed. Papers 146(2), 37–39 (2002)] and Nasiri [Journal of Clinical LaboRAtory Analysis 19:229–232 (2005)]for human peripheRAl blood samples.
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb ChakRAvart(Proteomic Center)
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb ChakRAvart(Proteomic Center)
免疫反应的细胞基础 - IRA Mellman P1
本视频由科普中国和生物医学大讲堂出品
IRA Mellman (Genentech) Part 1: Cellular Basis of the Immune Response
The immune system is charged with protecting us from invading microorganisms, a task that falls to a complex arRAy of highly specialized cell types spread throughout the body but that must work together as an integRAted system. How they accomplish and perform their functions can be wonderfully understood by probing the basic mechanisms governing their activities. In the first video, we will consider the oveRAll organization of the immune response in cellular terms, the innate immune system (comprising evolutionarily ancient phagocytic cells that recognize conserved molecules of microorganisms) and the adaptive immune system (composed of lymphocytes that recognize chemically diverse antigens).
抗原提呈和树突状细胞 - IRA Mellman Part 2
本视频由科普中国和生物医学大讲堂出品
早在100多年前，科学家就已经发现，免疫反应是由先天性和适应性免疫两个系统构成。而负责连接这两部分免疫反应的细胞类型，是最近才发现的树突状细胞。树突状细胞具有检测保守微生物产物的能力，可以激活细胞的先天免疫反应，并捕捉到广泛多样的微生物抗原抗体，也可以激活适应性免疫反应。抗原提呈和树突状细胞的独特能力，反映了细胞生物学的一系列显著的特化作用。
IRA Mellman (Genentech) Part 2: Antigen Presentation and Dendritic Cells
The immune response integRAtes two distinct systems of innate and adaptive immunity discovered over 100 years ago. Linking these two arms of the immune response is the task of a compaRAtively recently identified cell type, the dendritic cell. Dendritic cells have the capacity to detect the conserved microbial products that activate cells of the innate immune response and capture the dRAmatically wider diversity of microbial antigens to prime antibody and T cell responses chaRActeristic of adaptive immunity. The unique capacity of dendritic cells for antigen processing and presentation reflects a series of remarkable specializations of basic principles of cell biology.
下载生物谷APP，观看行云学院视频，让播放更流畅，使用更快捷！
生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：http://www.medsci.cn/m/
Controlling the Cell Cycle: Cdk SubstRAtes - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) Part 2: Controlling the Cell Cycle: Cdk SubstRAtes
Cyclin-dependent kinases (Cdks) are the centRAl components of the control system that initiates the events of the cell cycle. In the second part of this lecture, I discuss my laboRAtory's efforts to address the problem of how the Cdks trigger cell-cycle events. I describe our methods for identifying the protein substRAtes of the Cdks, and I discuss how these studies have led to important clues about how Cdks find their correct targets in the cell and how phosphorylation of those targets governs their function.
KRAS和肿瘤靶向治疗 - 陈巍学基因（36）
本视频介绍KRAS基因：
1、结构与功能；
2、在肿瘤中的变异；
3、对肿瘤靶向用药的指导作用；
4、设计针对KRAS基因的靶向药物的难点，和已取得的部分进展；
视频时长12分钟，建议在Wifi条件下观看：
Stability of Morphogen GRAdients & Movement of Molecules
In my second lecture I describe experiments using EGFP tagged Bicoid to follow Bcd gRAdient establishment in living embryos, and to test various aspects of the simple model. Despite continuous synthesis of new Bcd protein at the anterior end of the egg, we find that the concentRAtion of Bcd in nuclei at any given point along the anterior posterior axis is constant over time and is reproducible from embryo to the next. This reproducibility means that the gRAdient is sufficiently robust to provide positional information and thus can accuRAtely direct gene activities. One the other hand, quantitative imaging experiments point to seveRAl features of the gRAdient that are hard to explain - how target genes activated by Bcd distinguish relatively subtle differences in low concentRAtions, and how Bcd molecules move from the anterior site of their synthesis to the site of their tRAnscriptional activity. See more at http://www.ibioseminars.org
BRAF基因与黑色素瘤 - 陈巍学基因（38）
BRAF基因是细胞增殖信号调控中重要的一环。BRAF V600E突变是黑色素瘤中出现频率很高的致癌基因突变。威罗菲尼、达拦菲尼等靶向药物可以治疗有V600E突变黑色素瘤，但长期使用会产生耐药。本视频介绍了相关的知识。
CRISPR/Cas9 & TetRAOne：基因敲除/敲入鼠模型的快速构建技术
众所周知，基因工程小鼠模型已被广泛应用于生物医药研究，但模型小鼠的构建技术复杂、耗时长且花费高，让很多实验室望而却步。在本次讲座中，欧阳应斌博士（赛业生物技术副总裁、高级科学家）主要介绍基因敲除/敲入鼠模型的快速构建技术——CRISPR/Cas9基因编辑技术与TetRAOne技术，同时会简述转基因技术（PiggyBac系统）和传统ES打靶技术，并重点讲解每种技术的优势、缺点及应用。
PKI MantRA Intro 2015
The MantRA™ quantitative pathology workstation lets you visualize, quantify, and phenotype multiple types of immune cells simultaneously in intact FFPE tissue sections for cancer immunology research. MantRA allows you to maintain tissue morphology to help better understand the types and roles of cells within the tumor and tumor microenvironment. The system comes with powerful inForm® Tissue Finder™ advanced image analysis software that features user-tRAinable algorithms for automatic recognition of specific tissue and cell types, as well as per-cell analysis of biomarker expression and phenotyping to differentiate among cell types. Pathology Views™ creates familiar-looking brightfield (H&E, DAB, hematoxylin) renderings from original multi-label fluorescence images. PerkinElmer’s cancer immunology workflow has been validated using Opal™ reagent kits. Opal methodology enables specific staining of multiple tissue biomarkers reaching up to 6-plex and beyond in a single tissue section. Learn more: http://www.perkinelmer.com/catalog/pr...