Simultaneous quantification of 47 GEne expression in FFPE samples by a novel PCR-free approach
基因表达(GEne expression)是指细胞在生命过程中，把储存在DNA顺序中遗传信息经过转录和翻译，转变成具有生物活性的蛋白质分子。生物体内的各种功能蛋白质和酶都是同相应的结构基因编码的。差别基因表达(differential GEne expression)指细胞分化过程中，奢侈基因按一定顺序表达，表达的基因数约占基因总数的5%～10%。
Erich GnaiGEr：Life Style and Mitochondrial Competence – Modern Drugs for T2 Diabetes in Aging and DeGEnerative Diseases.
D. Swarovski Research Laboratory (Mitochondrial Physiology), Dept. GEneral, Visceral and Transplant SurGEry, Innsbruck Medical University; and OROBOROS INSTRUMENTS, Innsbruck, Austria. - Email: erich.gnaiGEr@oroboros.at
The contribution of mitochondrial dysfunction to the etiology of T2 diabetes and a ranGE of preventable metabolic diseases is the subject of intensive current research with world-wide health implications.
Recently these investigations gained depth and scope by technological advances for diagnosis of mitochondrial function by comprehensive OXPHOS analysis using high-resolution respirometry [1,2]. Fundamental questions of a causal relationship, however, between compromised mitochondrial function and development of T2 diabetes remain to be resolved [3,4] to optimize prevention and treatment of insulin resistance.
For preventable diseases such as T2 diabetes, the evolutionary background of mitochondrial competence provides a solid basis for improved and broad application of a well established modern drug, mtLSD.
Post-industrial societies are characterized by a high-energy input lifestyle with diminished physical activity and high incidence of non-transmittable diseases, in comparison to human populations where physical work is essentially important for sustaining life and in which deGEnerative diseases (T2 diabetes, various cancers, Alzheimer's) are essentially absent [5]. The capacity of oxidative phosphorylation (OXPHOS) is increased or maintained high by a life style involving endurance exercise and strength training [6].
Life style chanGEs from the aGE of 20-30 years to the elderly, but is subject to chanGE and intervention. Depending on group selection in cross-sectional studies, OXPHOS capacity declines from the aGE of 20-30 years [7,8], or is independent of aGE up to 80 years [9,10].
Independent of aGE, there is a strong decline of OXPHOS capacity in human vastus lateralis from BMI of 20 to 30 [1]. At a BMI >30, a threshold OXPHOS capacity is reached in human v. lateralis that may be characteristic of a low-grade inflammatory state (‘mitochondrial fever’).
Onset of deGEnerative diseases (T2 diabetes, neuromuscular deGEneration, various cancers) and mitochondrial dysfunction interact in an amplification loop progressing slowly with aGE, such that cause and effect of mitochondrial dysfunction cannot be distinguished. Diminished antioxidant capacity at low mitochondrial density is an important mechanistic candidate in the state of mitochondrial fever.
For implementing a life style supporting mitochondrial competence and preventing deGEnerative diseases in modern societies, we need (1) extended research programmes focused on the causative link between mitochondrial competence and effective prevention of deGEnerative diseases, (2) educational programmes on mitochondrial physiology tarGEted at GEneral practitioners, teachers and the society at larGE, (3) cooperation of health care and insurance organizations to support preventive life style activities, and (4) do not miss any opportunity in taking the lead in living the mtLife Style Drug (mtLSD).
GEnerating B-lymphoblastoid cell lines using Epstein Barr virus transformation.
GEnerating immortalized B-lymphoblastoid cell lines via Epstein Barr virus transformation using the B95-8 EBV-infected and producing marmoset cell line.
Western Blot Using The invitroGEn NuPAGE Novex Bis-Tris MiniGEl System(Aubin Penna.Ph.D)
Western Blot Using The invitroGEn NuPAGE Novex Bis-Tris MiniGEl System(Aubin Penna.Ph.D)
头足纲动物的伪装和信号 - RoGEr Hanlon P1
本视频由科普中国和生物医学大讲堂出品
RoGEr Hanlon (MBL) Part 1: CamouflaGE and Signaling in Cephalopods
Hanlon introduces the amazing adaptive coloration of cephalopods. He uses video and still photography to showcase their ability to rapidly chanGE color, pattern and skin texture with fine control and a diversity of appearances, to produce camouflaGE or to send signals. He argues that all camouflaGE patterns in nature can be grouped into three types. In part 2, Hanlon shows us results from his lab that make a convincing case that the rapid adaptive coloration of cephalopods is controlled by their visual system; quite impressive for a color-blind animal! Part 3 focuses on the unique skin of cephalopods including the system of pigments and reflectors that allows it to quickly chanGE to any hue and contrast, and the papillae musculature that allows the skin to deform and create multiple 3D textures.
对头足纲动物视觉感知机制的探索 - RoGEr Hanlon P2
本视频由科普中国和生物医学大讲堂出品
RoGEr Hanlon (MBL) Part 2: Exploring Mechanisms of Visual Perception
Hanlon introduces the amazing adaptive coloration of cephalopods. He uses video and still photography to showcase their ability to rapidly chanGE color, pattern and skin texture with fine control and a diversity of appearances, to produce camouflaGE or to send signals. He argues that all camouflaGE patterns in nature can be grouped into three types. In part 2, Hanlon shows us results from his lab that make a convincing case that the rapid adaptive coloration of cephalopods is controlled by their visual system; quite impressive for a color-blind animal! Part 3 focuses on the unique skin of cephalopods including the system of pigments and reflectors that allows it to quickly chanGE to any hue and contrast, and the papillae musculature that allows the skin to deform and create multiple 3D textures.
头足纲动物的可变化的皮肤细胞 - RoGEr Hanlon P3
本视频由科普中国和生物医学大讲堂出品
RoGEr Hanlon (MBL) Part 3: ChanGEable Skin
Hanlon introduces the amazing adaptive coloration of cephalopods. He uses video and still photography to showcase their ability to rapidly chanGE color, pattern and skin texture with fine control and a diversity of appearances, to produce camouflaGE or to send signals. He argues that all camouflaGE patterns in nature can be grouped into three types. In part 2, Hanlon shows us results from his lab that make a convincing case that the rapid adaptive coloration of cephalopods is controlled by their visual system; quite impressive for a color-blind animal! Part 3 focuses on the unique skin of cephalopods including the system of pigments and reflectors that allows it to quickly chanGE to any hue and contrast, and the papillae musculature that allows the skin to deform and create multiple 3D textures.
用SDS-PAGE技术分离蛋白
十二烷基硫酸钠聚丙烯酰胺凝胶电泳，也称SDS-PAGE，是一种被广泛使用，仅根据分子量大小来分离蛋白质混合物的技术。阴离子去污剂SDS，在变性的线性蛋白表面沿长度均匀分布使其带电。将它们上样到聚丙烯酰胺凝胶后，施加电压，这些表面覆盖SDS的蛋白将被分开。电场作为驱动力，牵引SDS结合的蛋白朝阳极移动，分子量大的蛋白将比小的蛋白移动慢。为了判断蛋白的大小，已知分子量大小的蛋白质标准也会和样品一起上样并在同等条件下跑胶。
本短片介绍SDS-PAGE技术,首先将解释其背后的原理，然后演示每一步的操作过程。视频还将讨论实验中的各种参数，如聚丙烯酰胺浓度，和用于跑胶的电压。还会介绍电泳之后的考马斯亮蓝和银染色方法，以及其他电泳技术，如双向凝胶电泳。