抗原提呈和树突状细胞 - Ira Mellman PARt 2
本视频由科普中国和生物医学大讲堂出品
早在100多年前，科学家就已经发现，免疫反应是由先天性和适应性免疫两个系统构成。而负责连接这两部分免疫反应的细胞类型，是最近才发现的树突状细胞。树突状细胞具有检测保守微生物产物的能力，可以激活细胞的先天免疫反应，并捕捉到广泛多样的微生物抗原抗体，也可以激活适应性免疫反应。抗原提呈和树突状细胞的独特能力，反映了细胞生物学的一系列显著的特化作用。
Ira Mellman (Genentech) PARt 2: Antigen Presentation and Dendritic Cells
The immune response integrates two distinct systems of innate and adaptive immunity discovered over 100 yeARs ago. Linking these two ARms of the immune response is the task of a compARatively recently identified cell type, the dendritic cell. Dendritic cells have the capacity to detect the conserved microbial products that activate cells of the innate immune response and capture the dramatically wider diversity of microbial antigens to prime antibody and T cell responses chARacteristic of adaptive immunity. The unique capacity of dendritic cells for antigen processing and presentation reflects a series of remARkable specializations of basic principles of cell biology.
下载生物谷APP，观看行云学院视频，让播放更流畅，使用更快捷！
生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：http://www.medsci.cn/m/
剪接体的结构和动力学 - Melissa Moore PARt 2
本视频由科普中国和生物医学大讲堂出品
本节课Melissa Moore讲述了，剪接体的成分和在每个工作周期中的剪接体。穆尔还解释了如何利用荧光蛋白标签和全反射显微镜的创新，使她和她的同事们能够更好地理解复接机器的有序组装与功能。
Melissa Moore (U. Mass/HHMI) PARt 2: Spliceosome Structure and Dynamics
Moore goes on to describe the cellulAR splicing machine, the spliceosome, in greater detail in PARt 2. She lists the components of the spliceosome and where each works in the spliceosome cycle. Moore also explains how the innovative use of fluorescent protein tags and total internal reflection microscopy has allowed her and her colleagues to better understand the ordered assembly and function of the complex splicing machine.
下载生物谷APP，观看行云学院视频，让播放更流畅，使用更快捷！
生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：http://www.medsci.cn/m/
Photoreceptors and Image Processing PARt 1A - Jeremy Nathans
本视频由科普中国和生物医学大讲堂出品
Jeremy Nathans (Johns Hopkins) PARt 1A: Photoreceptors and Image Processing
In this set of lectures, Jeremy Nathans explores the moleculAR mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing receptors, the intracellulAR signals that ARe triggered by light absorption, and the ways in which the retina extracts information from a complex scene. See more at http://www.ibioseminARs.org
Photoreceptors and Image Processing PARt 1B - Jeremy Nathans
本视频由科普中国和生物医学大讲堂出品
Jeremy Nathans (Johns Hopkins) PARt 1B: Photoreceptors and Image Processing
In this set of lectures, Jeremy Nathans explores the moleculAR mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing receptors, the intracellulAR signals that ARe triggered by light absorption, and the ways in which the retina extracts information from a complex scene. See more at http://www.ibioseminARs.org
布鲁斯·艾伯茨：LeARning from Failure
Alberts declARes "Success doesn't really teach you much, failure teaches you a lot." Speaking from his personal experience, Alberts asserts that all scientists make mistakes and suffer setbacks but leARning from those failures is what allows one ultimately to succeed.
GE：DhARmacon Edit-R基因编辑平台——即开即用，任性编辑！
CRISPR-Cas9基因编辑系统正以前所未有的速度席卷科研界。自从GE DhARmacon推出Edit-R CRISPR-Cas9系统以来，其"无需克隆，任性编辑"的特点广受好评。在此基础上，GE DhARmacon进一步扩充并更新了Edit-R基因编辑系统，以业界领先的guide RNA设计技术提供各种即开即用的预制产品，同时提供全基因组文库和各信号通路亚文库，满足不同实验者的实验需求。
GE：DhARmacon siRNA 文库产品应用解决方案
GE DhARmacon是siRNA全基因组文库的发明者，拥有RNAi产品领域最多的专利和相关知识产权。DhARmacon pre-defined siRNA文库是目前应用最广泛、客户最值得信赖的siRNA文库。同时，DhARmacon牵头成立的RNAi全球协议组织（RGI）的60多个知名科研院所成员使用DhARmacon siRNA文库取得了良好的效果。siRNA文库筛选加速了基因功能的研究，加深了疾病发生机理的研究，并获得了更多新的药物作用机理和潜在药物靶点，已经成为获得高质量研究数据和加速研究进程的必备工具。
Protein synthesis: a high fidelity moleculAR event
Rachel Green (Johns Hopkins U., HHMI) 1: Protein synthesis: a high fidelity moleculAR event
Talk Overview:
In her first talk, Green provides a detailed look at protein synthesis, or translation. Translation is the process by which nucleotides, the “language” of DNA and RNA, ARe translated into amino acids, the “language” of proteins. Green begins by describing the components needed for translation; mRNA, tRNA, ribosomes, and the initiation, elongation, and termination factors. She then explains the roles of these players in ensuring accuracy during the initiation, elongation, termination and recycling steps of the translation process. By compARing translation in bacteria and eukARyotes, Green explains that it is possible to determine which components and steps ARe highly conserved and predate the divergence of different kingdoms on the tree of life, and which ARe more recent adaptations.
Green’s second talk focuses on work from her lab investigating how ribosomes detect defective mRNAs and trigger events leading to the degradation of the bad RNA and the incompletely translated protein product and to the recycling of the ribosome components. Working in yeast and using a number of biochemical and genetic techniques, Green’s lab showed that the protein Dom34 is critical for facilitating ribosome release from the short mRNAs that result from mRNA cleavage. Experiments showed that Dom34-mediated rescue of ribosomes from short mRNAs is an essential process for cell survival in higher eukARyotes.
Speaker Biography:
Rachel Green received her BS in chemistry from the University of Michigan. She then moved to HARvARd to pursue her PhD in the lab of Jack Szostak where she worked on designing catalytic RNA molecules and investigating their implications for the evolution of life. As a post-doctoral fellow at the University of California, Santa Cruz, Green began to study how the ribosome translates mRNA to protein with such accuracy.
Currently, Green is a Professor of MoleculAR Biology and Genetics at the Johns Hopkins School of Medicine and an Investigator of the HowARd Hughes Medical Institute. ReseARch in her lab continues to focus on the ribosome and factors involved in the fidelity of eukARyotic and prokARyotic translation.
Green is the recipient of a Johns Hopkins University School of Medicine Graduate Teaching AwARd as well as the recipient for numerous awARds for her reseARch. She was elected to the National Academy of Sciences in 2012.
盐皮质激素受体通过调控miR-338-3p-PKLR轴抑制肝癌的发展和WARburg效应
激素和它们的受体在生理和病理条件下对代谢的调节起着重要的作用。我们在4株肝癌细胞用siRNA的方法筛选20种激素受体对肝癌细胞的瓦伯格效应（WARburg effect）尤其是乳酸产生的影响。我们发现很多受体的siRNA都影响乳酸的产生。其中盐皮质激素受体(mineralocorticoid receptor, MR) 的siRNA在4株肝癌细胞都表现出增加乳酸的产生。体外和体内实验表明MR影响细胞增殖、细胞周期和凋亡。进一步的机制研究揭示，作为一个转录因子，MR直接调节miR-338-3p的表达，而miR-338-3p又通过靶基因PKLR(pyruvate kinase, liver and red blood，糖酵解途径的关键酶)来抑制肝癌细胞的瓦伯格效应。另外，与癌旁组织相比，有81%的肝癌病人的肝癌组织中MR的表达都发生下调。这种下调是由MR的染色体缺失和去乙酰化引起的。在肿瘤组织中，MR的低表达和病人的预后差相关；miR-338-3p的表达和MR的表达水平呈正相关，和PKLR的表达呈负相关。结论：我们的研究首次揭示了MR通过miR-338-3p/PKLR这个途径抑制肝癌的瓦伯格效应。
PureBlu™ Hoechst 33342 NucleAR Staining Dye for Live Cells - A Fast Approach to Staining Nuclei
This brief tutorial demonstrates the use of the PureBlu Hoechst 33342 Dye with the ZOE™ Fluorescent Cell Imager for routine nucleAR staining in fluorescence microscopy and cell imaging applications.